An electrochemical ovalbumin immunosensor based on nanocellulose and MXene nano-famework Faheem Kareem, Minhaz Uddin Ahmed Universiti Brunei Darussalam, Brunei Darussalam A versatile and highly sensitive sensing platform based on nanocellulose (NCs) and MXene nanocomposite has been developed at the surface of glassy carbon electrode for ovalbumin (Ova) detection. Firstly, the dialdehyde group was introduced onto the surface of nanocellulose before being decorated with MXene nanosheet. Ovalbumin antibody was immobilized on the surface of functionalized NCs through the covalent bonding between amino groups of Ova and dialdehyde group of NCs. Moreover, the incorporation of MXene nanosheets can increase the electrochemical signal due to their high electrocatalytic characteristics, high specific surface area, outstanding electrical conductivity, and good biocompatibility. Key processing variables including antibody concentration, antibody binding time, pH, and time required for immunocomplex formation were optimized. Ova was electrochemically detected by using Differential Pulse Voltammetry (DPV) in 5mM K 3 Fe(CN 6 ) as a redox probe. The fabricated immunosensor exhibited higher sensitivity to Ova under optimal conditions, with a linear range of 0.01 pg/mL to 100 ng/mL and limit of detection (LOD) of 0.57 fg/ml. This novel immunosensor also demonstrated high selectivity, reproducibility, interference resistance and achieved excellent recoveries of Ova detection in spiked real food samples, displaying the potential applicability of the designed immunosensor. References 1. Shao, Y., et al., Two-dimensional MXene nanosheets (types Ti3C2Tx and Ti2CTx) as new ion-to-electron transducers in solid-contact calcium ion-selective electrodes. 2019. 186 (12): p. 1-9. 2. Zhang, Z., et al., Self-reduction synthesis of new MXene/Ag composites with unexpected electrocatalytic activity. 2016. 4 (12): p. 6763-6771.
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