HPLC-ICP-MS and post-column isotope dilution analysis of the Fe protein profile for differential diagnosis of stroke: application to nasal exudate and serum Marta Marina Latorre 1 , Marta Aranaz 1 , Claudia García-Revuelta 1 , Lara Lobo 1 , Héctor González-Iglesias 2 , Carmen García-Cabo 3 , Estefanía Costa-Rama 1 , M. Teresa Fernández-Abedul 1 and Rosario Pereiro 1 1 Department of Physical and Analytical Chemistry, University of Oviedo, Spain, 2 Department of Technology and Biotechnology of Dairy Products, IPLA-CSIC, Spain, 3 Central University Hospital of Asturias, Oviedo, Spain According to The World Health Organization (WHO) the cerebrovascular accident (CVA), or stroke, is the second cause of death worldwide. There is also a high morbidity associated with this pathology: about 50% of survivors end up chronically disabled. CVA can be classified as ischemic (caused by a blood clot) or hemorrhagic (produced by broken blood vessels), both causing a decrease in the blood flow to the brain. It is crucial a fast diagnosis of the CVA-type in order to start the proper treatment and thus to achieve a better recovery of the patients. Direct clinical parameters associated with the CVA in the brain is rather limited due to this organ is highly immunoprotected by the blood-brain barrier. However, the recent description of a lymphatic drainage from the brain to the nasal mucosa paves the way to investigate nasal exudate to provide information about CVA, being observed noticeable differences in total Fe concentration when comparing ischemic and hemorrhagic patients [1]. In this context, and aiming at the search of potential biomarkers allowing for a fast differential diagnosis of CVA, we have investigated the potential differences in the protein profile of Fe (this element is well known to be linked with CVA) between ischemic and hemorrhagic stroke patients. For such aim, a method based on size exclusion chromatography (SEC-HPLC) coupled to ICP-MS and quantitative analysis via post-column isotope dilution (IDA) has been developed for two types of biological fluids: serum (most common fluid used in diagnosis) and nasal exudate (as a source of direct information from the brain), and results compared for ischemic and hemorrhagic CVA patients as well as for controls. In addition, elemental analysis of iron concentrations has been carried out for both biological fluids. References 1. C. García-Cabo, P. Llano-Suarez, Clin. Chem. Lab. Med., 2019 , 58, 847-853.
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