01 - Forensic Escape Room: Design Your Own Biotech Adventure
Module III: DNA Fingerprinting, continued
RUNNING THE GEL 9.
REMINDER: Before loading the samples, make sure the gel is properly oriented in the
PLACE gel (on the tray) into electrophoresis chamber. COVER the gel with 1X electrophoresis buffer (See Table B for recommended volumes). The gel should be completely submerged. 10. PUNCTURE the foil overlay of the QuickStrip™ with a pipet tip. LOAD the entire sample (35 µL) into the well as indicated by the Gel Loading table.
apparatus chamber.
11. PLACE safety cover. CHECK that the gel is properly oriented. Re- member, the DNA samples will migrate toward the positive (red) electrode. 12. CONNECT leads to the power source and PERFORM electrophoresis. (See Table C for time and voltage guidelines.) 13. After electrophoresis is complete, REMOVE the gel and casting tray from the electro- phoresis chamber.
GEL LOADING TABLE
VISUALIZING the SYBR® GEL SLIDE gel off the casting tray onto the viewing surface of the transilluminator. TURN the unit on. DNA should appear as bright green bands on a dark background. PHOTOGRAPH results.
SAMPLE A B C D
LANE 1 2 3 4
SAMPLE NAME DNA standard marker Crime scene sample Deena PCR Reaction Monica PCR Reaction
Time and Voltage Guidelines (0.8% Agarose Gel)
1x Electrophoresis Buffer (Chamber Buffer)
EDGE™
M12 & M36
Volts
Min/Max (minutes)
Min/Max (minutes)
EDGE™
150 mL
3 mL
147 mL
150 125 100
10/20
20/35 30/45 40/60
M12
400 mL
8 mL
392 mL
N/A
M36
1000 mL
20 mL
980 mL
15/25
11
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