01 - Forensic Escape Room: Design Your Own Biotech Adventure
Module III: DNA Fingerprinting, continued
8. PLACE the gel (still on the tray*) into the electrophoresis chamber. COVER the gel with 1X Electrophoresis Buffer (See Table B for recommended volumes). The gel should be com- pletely submerged. 9. PUNCTURE the foil overlay of the QuickStrip™ with a pipet tip. LOAD the entire sample (35
TUBE A B C D
LANE 1 2 3 4
SAMPLE NAME DNA standard marker Crime scene blood collected from knife Laura PCR Reaction Sarah Ann PCR Reaction
µL) into the well in the order indicated by the Table, at right. 10. PLACE safety cover on the unit. CHECK that the gel is properly oriented. Remember, the DNA samples will migrate toward the positive (red) electrode.
11. CONNECT leads to the power source and PERFORM electrophoresis (See Table C for time and voltage guidelines). Allow the tracking dye to migrate at least 3 cm from the wells. 12. After electrophoresis is complete, REMOVE the gel and casting tray from the electrophore- sis chamber and PROCEED to gel staining.
Time and Voltage Guidelines (0.8% Agarose Gel)
1x Electrophoresis Buffer (Chamber Buffer)
EDGE™
M12 & M36
Volts
Min/Max (minutes)
Min/Max (minutes)
EDGE™
150 mL
3 mL
147 mL
150 125 100
10/20
20/35 30/45 40/60
M12
400 mL
8 mL
392 mL
N/A
M36
1000 mL
20 mL
980 mL
15/25
11
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