01 - Forensic Escape Room: Design Your Own Biotech Adventure
Experimental Results and Analysis
MODULE I ANALYSIS
Negative Sample
Positive Sample
CS1 was negative and did not produce any color change after adding H 2 O 2 , while CS2 and CS3 turned bright pink and were posi- tive. Therefore, CS2 and CS3 should continue on for confirmatory testing.
MODULE II ANALYSIS
The crime scene sample CS2 is type O and crime scene sample CS3 is type B blood. Both Deena and Monica are type B. While blood typing can narrow down suspects, DNA testing would have to be
performed to conclusively say if the blood belongs to either of the two suspects. Therefore, PCR samples from crime scene sample CS3 should be DNA tested alongside PCR samples from Monica and Deena.
MODULE III ANALYSIS
The DNA standards in Lane 1 make it possible to measure the DNA bands obtained from the PCR reactions. The results of this analysis indicates an identical pattern in Lanes 2 and 4. This is strong evidence that the crime scene DNA matches the sample from Monica. In criminal investigations, several known variable regions in DNA are analyzed to match crime scene and suspect DNAs.
TUBE A B C D
LANE 1 2 3 4
SAMPLE NAME DNA standard marker Crime scene sample Deena PCR Reaction Monica PCR Reaction
MOLECULAR WEIGHTS 6751, 3652, 2827, 1568, 1118, 825, 630 3000, 1282 3000 3000, 1282
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