02 - Code Breakers: Using CRISPR to Rewrite Genetics
Experiment Results and Analysis
WRITE YOUR HYPOTHESES BELOW: Set 1: E coli Chomopurple + pCas-purple
The sgRNA is designed to bind to and inactivate the mRNA for the purple chromoprotein. The mRNA will not be able to be translated into purple protein so the colonies will be white. Set 2: E coli Chomopurple + pCas-blue: The sgRNA is designed to bind to and inactivate the mRNA for the beta-gal enzyme. Because it is not present in these cells, the sgRNA-Cas complex cannot cut the mRNA. The colonies will be purple. Set 3: E coli HB101 + pCas-blue: The sgRNA is designed to bind to and inactivate the mRNA for the beta-gal enzyme. The mRNA will not be able to be translated into the enzyme to create the blue color so the colonies will be white. Set 4: E coli HB101 + pCas-purple: The sgRNA is designed to bind to and inactivate the mRNA for the purple chromoprotein. Because it is not present in these cells, the sgRNA-Cas complex cannot cut the mRNA. The colonies will be blue.
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