04 - Put the M into STEM: Quantitative Techniques for Biotechnology
Experimental Results and Analysis
Electrophoresis Results:
Lane
Tube
Sample
Molecular Weights (in bp)
1
A
DNA Standard
6751, 3652, 2827, 1568
Markers
1118, 825, 630
2
B
0 cycles
No bands
3
C
5 cycles
2999
4
D
10 cycles
2999
5
E
20 cycles
2999
6
F
30 cycles
2999
ELISA Results: The color intensity of each well reflects the initial concentration of whey. This ELISA experiment demonstrates the quantification of varying concentrations of antigens as detected by the inten - sity of the color reaction due to the accumulation of products. We prepared serial dilutions to determine the concentration of two antigens through comparison. A STEM extension quantifies the data using image analysis software.
1
2
3
4
C
E
Well
1
2
3
4
Dilution
---
1:4
1:8
0
Concentration
20
5
2.5
0
µg/mL
µg/mL
µg/mL
µg/mL
50
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