05 - Heavy Metal: Effects of Environmental Toxins on C. elegans
21. To collect data for the effects of the control solution on the wild-type strain, REPEAT steps 11 through 19 using the wild-type strain and your C-WT labeled items. RECORD your results in Table 2. 22. To collect data for the effects of the control solution on the mutant strain REPEAT steps 11 through 19 using the mutant strain and your C-MS labeled items. RECORD your results in Table 2. Module III: Collection of C. elegans and Toxicity Bioassay Excerpts from Edvo-Kit #856
ild Type ash Count 30 sec.) Mutant Strain Thrash Count (30 sec.) y Metal_____________________
TABLE 2: Control Solution
Wild Type Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
0 min.
2 min.
4 min.
TABLE 1: Heavy Metal_____________________ TABLE 1: Heavy Metal_____________________
TABLE 2: Control Solution
TABLE 2: Control Solution
Wild Type Thrash Count (30 sec.)
Wild Type Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
Wild Type Thrash Count (30 sec.)
Wild Type Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
ild Type ash Count 30 sec.) Mutant Strain Thrash Count (30 sec.) y Metal_____________________
TABLE 4: Heavy Metal_____________________
23. (Optional) If class time permits, TEST the other two provided heavy metals. Four test tubes, counting chambers, and small transfer pipets are provided. LABEL theses items, RECORD the selected heavy metals, and REPEAT steps 11 through 19 with these two new solutions on both strains. RECORD the results in Tables 3 and 4. Note that the control does not need to be repeated. 0 min. 2 min. 0 min. 2 min. Wild Type Thrash Count (30 sec.) Mutant Strain Thrash Count (30 sec.) 0 min. 0 min. 2 min. 0 min. 2 min.
2 min.
4 min.
4 min.
4 min.
4 min.
4 min.
TABLE 3: Heavy Metal_____________________ TABLE 3: Heavy Metal_____________________
TABLE 4: Heavy Metal_____________________ TABLE 4: Heavy Metal_____________________
Wild Type Thrash Count (30 sec.)
Wild Type Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
Wild Type Thrash Count (30 sec.)
Wild Type Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
Mutant Strain Thrash Count (30 sec.)
0 min.
0 min.
0 min.
0 min.
2 min.
2 min.
2 min.
2 min.
4 min.
4 min.
4 min.
4 min.
24. (Optional) GRAPH the change in thrash rate over time for each condition you tested (solution and strain combination) with time on the x-axis and thrash rate on the y- axis. 25. (Optional) SHARE your group’s findings with another lab group or with the class.
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