06 - Introducing Your Students to CRISPR with Sickle Cell Gene Editing
Experimental Results and Analysis
MODULE I There are eight PAM sites in the sequence, highlighted in the sequence below. Note, there are three potential PAM sites in the “GGGGA” DNA sequence: GGG, GGG, and AGG.
PAM Sequence
Sample Name
Target Sequence (spacer)
gRNA #1 gRNA #2 gRNA #3 gRNA #4 gRNA #5 gRNA #6 gRNA #7 gRNA #8
CTTTTTCTGTTAAAACATCT AAGCTGTGTCTGTAAACTGA TAAACTGATGGCTGCCCCTC TTCAGTCAAGTTTGCCTGAG GTTCAGTCAAGTTTGCCTGA AGTTCAGTCAAGTTTGCCTG TCAGGCAAACTTGACTGAAC AACTGGATATATATTCAAGA
AGG TGG AGG GGG GGG AGG TGG AGG
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