2024 NSTA New Orleans • EDVOTEK® Workshops

06 - Introducing Your Students to CRISPR with Sickle Cell Gene Editing

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For 8 Gels. In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an un - derstanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment. Cat. #135 Using CRISPR To Treat Cystic Fibrosis

EDGE™ Integrated Electrophoresis System Runs one 10 x 7 cm gel Cat# 500

M12 Complete™ Electrophoresis Package For 1 or 2 Lab Groups Cat# 502-504

DuoSource™ 100/150 V, for 1 or 2 Units Cat# 509

QuadraSource™ 10-300 V For 1 or 4 Units Cat# 5010-Q

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EDVOTEK® Variable Micropipette 5-50 µL Micropipette Cat. # 590

Fixed Volume MiniPipet™ 35 µL MiniPipet™ Cat. # 587-2

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