01 - Forensic Escape Room: Design Your Own Biotech Adventure
Module I: Kastle-Meyer Test
The first steps of your forensic analysis will use the Kastle-Meyer test as a presumptive test for blood. You will test the three crime scene samples to see if they are positive or negative for the presence of blood.
Hydrogen peroxide
2 drops evidence
2 drops ethanol
Phenolphthalein solution
1. Use the transfer pipet to ADD two drops, or 40 µL of blood evidence to the swab.
2. Use a transfer pipet to ADD two drops, or 40 μL, of 95% ethanol to the swab. NOTE any color change. PLACE the pipet and remaining ethanol to the side for testing additional samples.
3. Use a new pipet to ADD two drops, or 40 μL, of the phenolphthalein solution to the swab. NOTE any color change. No color change is expected even if blood is present. PLACE the pipet and remaining phenolphthalein to the side for testing additional samples. 4. Use a new pipet to ADD two drops, or 40 μL, of hydrogen peroxide to the swab. NOTE any color change. An immediate pink color is expected if blood is present. RECORD your results in the chart below.
REPEAT steps 1-4 for each blood evidence sample.
5.
Crime Scene Sample #
Positive or Negative for Blood
CS1
CS2
CS3
8
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