01 - Forensic Escape Room: Design Your Own Biotech Adventure
Module I: Kastle-Maeyer Test
The first steps of your forensic analysis will use the Kastle-Meyer test as a presumptive test for blood. You will test the three crime scene samples to see if they are positive or negative for the presence of blood.
25 µL evidence
50 µL Phenolphthalin solution
50 µL Hydrogen peroxide solution
50 µL ethanol
1. Use a transfer pipet to ADD 1 drop (or 25 µL) of blood evidence to the swab. NOTE: Using more than one drop (25 µL) of evidence may affect the results.
2. Use a transfer pipet to ADD 2 drops (or 50 µL) of 95% ethanol to the swab. NOTE any color change. PLACE the pipet and remaining ethanol to the side for testing additional samples. 3. Use a new pipet to ADD 2 drops (or 50 µL) of the phenolphthalin solution to the swab. NOTE any color change. No color change is expected even if blood is present. PLACE the pipet and remaining phenolphthalin to the side for testing additional samples.
IMPORTANT For steps 2-4: When adding the detection reagents, hold the evidence swab at a steep angle, with the crime scene sample at the bottom.
4. Use a new pipet to ADD 2 drops (or 50 µL) of hydrogen peroxide solution “H2O2” to the swab. NOTE any color change. A pink color is expected after several seconds if blood is present. RECORD your results in the chart below.
REPEAT steps 1-4 for each blood evidence sample.
5.
Crime Scene Sample #
Positive or Negative for Blood
CS1
CS2
CS3
8
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