07 - Lion Family Reunion: Conservation Biology Genetics
Visualizating the SYBR® Gel
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1. SLIDE the gel off the casting tray onto the viewing surface of the transillumina - tor and turn the unit on. ADJUST the brightness to the desired level to maximize band visualization. DNA should appear as bright green bands on a dark back- ground. 2. PHOTOGRAPH the results. 3. REMOVE and DISPOSE of the gel and CLEAN the transilluminator surfaces with distilled water.
Related Experiment:
Safari Family Reunion For 8 Gels/8 Lab Groups. In this lab, students will perform electrophoresis on the DNA samples of two lions in order to return them to wildlife sanctuaries close to their ancestral home. • Learn how DNA is used to discover an individual’s ancestry. • Perform DNA electrophoresis and RFLP analysis. • Analyze phylogenetic tree and haplotype maps. • Explore how conservation biologists use genetic data.
Cat. #920 Safari Family Reunion with FlashBlue™ Stain Cat. #920-S Safari Family Reunion with SYBR® Safe Stain
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