01 - Forensic Escape Room: Design Your Own Biotech Adventure
Module II: Blood Type Test
Now that some samples from the crime scene have been identified as likely blood using the Kastle-Meyer test, it is necessary to confirm the identity of the samples. In this Module, you will perform ABO testing on the blood samples from the crime scene. Note that Jeremy is blood type O, Sarah Ann is blood type A, and Laura is blood type A.
PLACE a microtiter plate piece as shown below.
1.
2. ADD 50 μL (or two drops from a transfer pipet) of each control blood type sample into each of the two corresponding wells. For example, control A blood type goes into the two wells under the letter “A”. Repeat the same procedure for the control blood types B, AB, O, and the two positive blood samples from the crime scene. Each well requires 50 μL.
3. Use a new pipette tip to ADD 50 μL of Anti-A serum into each of the wells in row #1. The same tip or transfer pipet can be used for all samples in row #1.
4. Use a new pipette tip to ADD 50 μL of Anti-B serum into each of the wells in row #2. The same tip or transfer pipet can be used for all samples in row #2.
5. Let the samples INCUBATE undisturbed on the lab bench for 5-10 minutes.
6. COMPARE the crime scene evidence with the control blood samples.
9
1.800.EDVOTEK • www.edvotek.com • info@edvotek.com
Made with FlippingBook flipbook maker