01 - Forensic Escape Room: Design Your Own Biotech Adventure
Module II: Blood Type Test
Now that some samples from the crime scene have been identified as blood using the Kastle-Meyer test, it is necessary to confirm the blood type of the samples. In this module, you will perform ABO testing on the blood samples from the crime scene. This will allow us to determine which crime scene blood samples are from the victim, and which samples were from the suspects. NOTE: Bryce is blood type O, Deena is blood type B, Alex is blood type A, and Monica is blood type B.
1. PLACE a microtiter plate piece as shown below. Using a permanent marker, label the plate. The column labels will include the four control blood types (A, B, AB, O) and any Module I crime scene samples that test positive for blood. The row labels will be Anti-A and Anti-B.
A B AB O
Anti A
Anti B
2. ADD 50 μL or two drops of each control blood type sample into each of the two corre - sponding wells. For example, control blood type A goes into the two wells under the letter “A”. Repeat the same procedure for the control blood types B, AB, O, and the two positive blood samples from the crime scene. Each well requires 50 μL. 3. Use a new pipette tip to ADD 50 μL or 2 drops of Anti-A serum into each of the wells in row #1. The same tip or transfer pipet can be used for all samples in row #1. 4. Use a new pipette tip to ADD 50 μL or 2 drops of Anti-B serum into each of the wells in row #2. The same tip or transfer pipet can be used for all samples in row #2. 5. Firmly HOLD the plate piece on the lab bench as shown. Very gently TAP the side of the plate with your finger near the positive crime scene samples to MIX for approximately 20- 30 seconds. Do this VERY CAREFULLY to avoid spilling any of the samples from the wells. PROCEED to the next step. 6. To visualize the results, PLACE the microtiter plate on a white light transilluminator, or use a bright flashlight held above the plate. COMPARE the crime scene evidence with the control blood samples. RECORD your results.
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