S2514
Radiobiology - Biology of novel irradiation techniques
ESTRO 2026
(necrotic regions; Spearman r = –0.64), although these correlations did not reach statistical significance. A comprehensive marker analysis pipeline was developed to assess spatial colocalization of hypoxia with additional tumor features. Heatmaps were generated for cytoplasmic markers CD44 and CD98 (putative tumor stem cell markers) and mitochondrial super-oxide dismutase 2 (SOD2/Mt) to evaluate mitochondrial oxidative stress response. Conclusion: The established QuPath-based analysis pipeline enables integrated assessment of tumor oxygenation, perfusion, and molecular markers. It will be applied to an expanded dataset to further investigate stem cell composition and mitochondrial responses to treatment. Keywords: proton FLASH, HNSCC, in vivo pO2
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