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Training in Pulsed-Field Gel Electrophoresis GENOMICS

GENOMICS

MiSeq Training

Pulsed-field gel electrophoresis (PFGE) is a laboratory technique to separate large DNA molecules by applying an electric field that periodically changes direc- tion to a gel matrix. This course explains the PFGE technique, enabling users to successfully use it independently. Topics covered include: • Gel and Sample Preparation • Machine setup (BCL standard program only, users to define their own proto- cols for larger size separation) • Staining and Imaging using the Gel Doc System PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who are involved in genom- ics research and sequencing high molecular weight targeted fragments of DNA. PREREQUISITES • Active Badger account • Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training • User-provided DNA samples with a preferred DNA concentration of 100ng in 2 µL total MAXIMUM PARTICIPANTS 3 DURATION 2 days, 4 hours each day FREQUENCY Monthly

MiSeq is a high throughput Next-Generation Sequencing system from Illumina. The sequencing process takes place on a flow cell with one lane. Cluster gener- ation, sequencing, and analysis are all done on the instrument itself. The se- quencing is based on Illumina SBS (Sequencing by Synthesis) chemistry. It gen- erates a maximum of 15 Gb data from ~25 million reads in 3 days when used in the 2x300 bp configuration. MiSeq is mostly suited for amplicon sequencing, small genome sequencing, and 16S/18S metagenomics sequencing. This training will provide participants with an overview of the instrument, its run parameters, and metrics. Upon completion, users will be able to prepare the sample for loading and to set up a sequencing run. This training is required for independent use of the instrument. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who are involved in genom- ics research and nucleic acid sequencing. PREREQUISITES • Active Badger account

DURATION 2 hours (not including run time) FREQUENCY Every 3 weeks

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training • Prepared, qualified, and quantified library/pool with minimum 2nM concentration MAXIMUM PARTICIPANTS 3

Learn Develop Excel

Specialized Instrument & Method Training Catalog

Edition 1, Sep te mber 2020

103

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