Core Labs Training_Catalogue-v2 (edit 2)-web

qPCR GENOMICS

GENOMICS

10X Genomics

Quantitative polymerase chain reaction (qPCR) is a molecular biology technique used to quantify nucleic acid. The amplification via PCR of the target molecule is followed in real-time using fluorophores that either intercalate in the PCR product or are activated during the amplification process. The increase of fluorescence measured at each PCR cycle is used to determine nucleic acid concentration. This training provides users with an understanding of operation procedures, enabling them to successfully use the qPCR platforms independently. Sample preparation and loading, as well as result analysis will be covered. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who are planning to use qPCR. PREREQUISITES • Active Badger account

Single cell transcriptomics allows for the detection of mRNA transcripts from 500 to 10000 single cells. 10X Genomics is the leading platform in the field. The course will guide users through the various aspects of sample preparation and introduce the instrument’s workflow. It presents both theory and hands-on training with user-provided samples. The trainee will also be familiarized with a sample data report. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who are studying single cell transcriptomics in mammalian cells. Basic knowledge of Molecular Biology is required. PREREQUISITES • Active Badger account

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 2 DURATION 1 hour theory 12 hours practical training

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 3 DURATION 3 hours (not including run time) FREQUENCY Monthly

1 hour data report FREQUENCY Every 3 weeks

Learn Develop Excel

Specialized Instrument & Method Training Catalog

Edition 1, Sep te mber 2020

105

Made with FlippingBook - Online catalogs