Core Labs Training_Catalogue-v2 (edit 2)-web

Illumina Library Preparation Training GENOMICS

GENOMICS

Flow Cytometry Training

Library preparation is a critical step for Illumina sequencing and requires the generation of a pool of DNA fragments carrying specific adapters at both ends. Preparing a library for NGS involves nucleic acid isolation, fragmentation (where needed), adapter ligation, and amplification. Preparation of libraries is similar for all Illumina platforms. The aim of this training is to explain the steps and pitfalls in gDNA/RNA library preparation. A sample QC will be provided, and Library QC will be discussed. Upon completion, participants will understand the principles of Illumina library preparation and be able to successfully prepare genomic DNA/RNA libraries independently. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who are involved in Illumina sequencing. PREREQUISITES • Active Badger account

Flow cytometry allows for the measurement of physical and fluorescent charac- teristics of particles/cells in a fluidic stream. Particles/cells ranging in size from 0.3 µm to 40 µm can be assessed quantitatively for high throughput analysis. This course covers the theory and practical training in flow cytometry. Partici- pants will learn about instrument setup and receive hands-on training with refer- ence beads and test samples. Upon completion of the course, users will be able to work independently on flow cytometry instruments. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who are studying the pheno- typic biology of prokaryotic and eukaryotic organisms. PREREQUISITES • Active Badger account

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training • Read-through of the BD FACS Canto II SOP soft copy MAXIMUM PARTICIPANTS 4 DURATION 2 hours theory 4 hours practical training FREQUENCY Twice monthly

DURATION 2 days FREQUENCY Every 3 weeks

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training • User-provided gDNA/RNA samples (or test sam- ples from BCL) with minimum gDNA/RNA amount of 100ng in 50 µL • DIN of gDNA samples or RIN of RNA samples over 8 MAXIMUM PARTICIPANTS 3

Learn Develop Excel

Specialized Instrument & Method Training Catalog

Edition 1, Sep te mber 2020

109

Made with FlippingBook - Online catalogs