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PROTEOMICS

PROTEOMICS

Protein Expression and Purification Training

Introduction to Shotgun Proteomics

Preparation of recombinant protein is one of the essential techniques in the field of biological sciences. This training gives an introduction to protein expression in E. coli systems and purification using the Fast Protein Liquid Chromatography (FPLC) system. Participants will become familiar with the basics of protein expression and pu- rification techniques; understand the procedure of protein expression in E. coli cells; and become familiar with the use of liquid chromatography using FPLC system. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who want to conduct protein expression and purification experiments. PREREQUISITES • Active Badger account

Shotgun proteomics, also known as bottom-up proteomics, is a technique that identifies proteins in complex mixtures using a combination of High Performance Liquid Chromatography (HPLC), also known as LC and Mass Spectrometry (MS). This training will introduce the most commonly used proteomics workflows, including peptide sample preparation, LC separation, and analysis by Tandem Mass Spectrometry (MS/MS). Data acquisition and MS control software will also be introduced.

PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who want to become familiar with shotgun proteomics in order to optimize their sample preparation and under-

stand data acquisition. PREREQUISITES • Active Badger account

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 2 DURATION 1 day FREQUENCY Monthly

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 2 DURATION 4 days FREQUENCY Monthly

Learn Develop Excel

Specialized Instrument & Method Training Catalog

Edition 1, Sep te mber 2020

121

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