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PROTEOMICS

PROTEOMICS

Introduction to LC-MS Analysis of Proteins

SpectraMax Plate Reader Training

SpectraMax M5e is a multi-mode plate reader that can measure UV as well as visible absorbance, luminescence, and fluorescence. This training explains how to operate the instrument. Participants will gain an understanding of the system operation, and how to adjust the settings according to their requirements. Plate format settings and data export will also be covered.

Intact protein analysis using a combination of Liquid Chromatography (LC) and Mass Spectrometry (MS) provides accurate mass measurement of a protein, the relative abundance of its isoforms, and the extent of post-translational modifica- tions. This course will introduce the use of LC-MS analysis of intact proteins on a high-resolution MS instrument, as well as basic data acquisition and deconvo- lution tools for molecular weight determination. Upon completion of the course, participants will be able to apply LC-MS to a particular research question.

PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who want to use the Spec-

PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry, who want to become familiar with mass spectrometric analysis of intact proteins in order to assess purity and

tramax Plate Reader. PREREQUISITES • Active Badger account

verify molecular weight. PREREQUISITES • Active Badger account

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 4 DURATION 1 hour FREQUENCY Monthly

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 2 DURATION 1 day FREQUENCY Monthly

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Specialized Instrument & Method Training Catalog

Edition 1, Sep te mber 2020

123

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