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Transmission Electron Microscopy – Level 1 ELECTRON MICROSCOPY

ELECTRON MICROSCOPY

Transmission Electron Microscopy – Level 2

PARTICIPANT PROFILE

This is an advanced-level training course that will allow researchers with basic TEM experience to operate Titan CT and Titan ST transmission electron microscopes (TEM). Users will learn how to deal with electron lenses and optics, figure out the types of contrasts in TEM-images, and understand Z-contrast STEM. They will be presented with the Kinetic theory of electron diffraction, Dynamical theory electron diffraction, and Theory of high-resolution TEM and STEM. Radiative and non-radiative transitions in materials induced by electron beams will also be discussed. After taking this course, researchers will be able to operate the microscope for ele- mental composition and mapping, and be able to acquire: bright-field TEM, dark-field TEM, selected area electron diffraction, high-resolution TEM, bright-field scanning TEM (BF-STEM), dark-field scanning TEM, high-resolution scanning TEM, X-ray energy dispersive spectroscopy (EDS), energy-fil- tered TEM (EFTEM), and electron energy loss spectroscopy (EELS). Transmission Electron Microscopy – Level 2 is conducted in three distinct training cours- es:

This course is intended for students, researchers, scien- tists, and engineers who want to understand the morphology and crystal structure of nanomate- rials from either life sciences or materials sciences at an ad- vanced level. PREREQUISITES • Active Badger account • Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Pre- paredness Training • LN2 Training • T12 Qualification MAXIMUM PARTICIPANTS 5 DURATION 12 hours, divided in 3-4 ses- sions FREQUENCY Monthly

Transmission electron microscopy is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. This course is an entry-level training that allows researchers of all backgrounds to operate Tecnai 12 transmission electron microscope (TEM). Users will learn about the constraints of working with high vacuum systems, about electron sources and high voltage, and about different types of contrasts in TEM-images. They will be given an understanding of electron optics and lenses and electron scattering from atoms and materials. Analysis of electron diffraction patterns will also be discussed. After taking this course, researchers will be able to independently operate the TEM Tecnai 12 to acquire bright-field TEM, selected area electron diffraction, and high-resolution TEM of crystalline materials with relatively large unit cells.

PARTICIPANT PROFILE This course is intended for students, researchers, scientists, and engineers who want to understand the morphology and crystal structure of nanomaterials in life sciences or materials science. PREREQUISITES • Active Badger account • Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training • LN2 Safety Training

MAXIMUM PARTICIPANTS 5 DURATION 12 hours, divided into 3-4 sessions FREQUENCY Monthly

• Advanced TEM • Analytical STEM • Analytical EFTEM

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Specialized Instrument & Method Training Catalog

Edition 1, Se p te mber 2020

175

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