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STED Superresolution Microscope Training OPTICAL MICROSCOPY

OPTICAL MICROSCOPY

Airyscan Microscope Training

Superresolution microscopy using stimulated emission depletion (STED) cre- ates sub-diffraction limit features by altering the effective point spread function of the excitation beam, and using a second laser that suppresses fluorescence emission from fluorophores located away from the center of excitation. The suppression of fluorescence is achieved through stimulated emission that occurs when an excited-state fluorophore encounters a photon that matches the energy difference between the ground and excited state. Upon interaction of the photon and the excited fluorophore, the molecule is returned to the ground state through stimulated emission before spontaneous fluorescence emission can occur. Thus, the lateral resolution can be improved to 50 nm.

With Airyscan, you collect all precious emission light without compromise, and get a unique combination of gentle superresolution imaging and high speed. Use multicolor samples with any label and get image quality like you’ve never seen before. You get perfect optical sections and resolve structures of 120 nm (in x, y) and 350 nm (in z) even in thick samples. Plus, Airyscan gives you an instant 4-8× improvement of signal-to-noise (SNR). You will also be harnessing this unique combination of greater SNR and resolution at increased acquisition speeds of 27 fps (at 480 x 480 pixels).

PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry. General knowledge of the

PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry. General knowledge of the

technique is required. PREREQUISITES • Active Badger account

technique is required. PREREQUISITES • Active Badger account

DURATION 5 days, 3 hours each day FREQUENCY Twice weekly

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training • Confocal Microscope Operation MAXIMUM PARTICIPANTS 4

• Confocal Microscope Operation MAXIMUM PARTICIPANTS 4 DURATION 2 days, 3 hours each day FREQUENCY Twice weekly

Learn Develop Excel

Specialized Instrument & Method Training Catalog

Edition 1, Se p te mber 2020

185

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