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ORGANICS

Dynamic Headspace Gas Chromatograph Flame Ionization-Thermal Conductivity and Mass Spectrometry Detectors ORGANICS

High-performance Liquid Chromatography (HPLC) Fractionation Coupled with Photodiode Array and Florescence Detection

The gas chromatograph is coupled with a dynamic headspace sample intro- duction device, and both a flame ionization detector and a mass spectrometry detector. It is particularly useful for the separation, structural elucidation, and quantification of volatile and semi-volatile organic compounds. This course in- troduces the general theory of chemical separation and column choices, includ- ing a discussion of the strengths and weaknesses of this method and general guidance for its applications. This training also includes discussions on sample preparation, instrument configuration, method development principles, and software usage. Following this course, the user is expected to be able to inde- pendently operate the instrument and perform basic data analysis. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry. PREREQUISITES • Active Badger account

High-performance-liquid-chromatography (HPLC) is coupled with UV and fluo- rescence detectors for separation and quantitative analysis in complex sample matrices. This instrument is capable of fractionating and purifying analytes from a complex mixture. This course introduces the general theory of chemical separation, columns, and mobile phases selection, including a discussion of the strengths and weaknesses of this method and general guidance for its applica- tions. This training also includes discussions on sample preparation, instrument configuration, method development principles, and software usage. Following this course, the user is expected to be able to independently operate the instru- ment and perform basic data analysis. PARTICIPANT PROFILE This course is intended for students and research professionals from KAUST, and their collaborators from academia and industry. PREREQUISITES • Active Badger account

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 2 DURATION 6 hours FREQUENCY Twice weekly

• Laboratory Safety Training • Hazardous Waste Training • Emergency Incident Preparedness Training MAXIMUM PARTICIPANTS 1 DURATION 7 hours FREQUENCY Twice weekly

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Specialized Instrument & Method Training Catalog

Edition 1, S e pt e mber 2020

51

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