03 - BioArt: Exploring Transformation with STEAM
Experiment Results and Analysis
-DNA plated with control cells (no DNA) Result: No colored cells visible. White colonies. May look like a smeared layer of cells. Demonstrates: Host bacterial cells are viable in the absence of ampicillin.
-DNA/+Amp plated with control cells (no DNA)
+DNA/+Amp/+IPTG plated with transformed
+DNA/+Amp plated with transformed cells (Rainbow Trans- formation Mixture)
cells (Rainbow Transformation Mixture)
Result: No colonies visible.
Result: White colonies.
Result: Individual pink, blue, and purple colonies. Demonstrates: Cells are resistant to Ampicillin when transformed with the mixture of three plasmids (pChromoBlue, pChromoPink, pChromo- Purple). Production of chromogenic protein is turned on in the presence of IPTG.
Demonstrates: Untransformed cells are sensitive to ampicillin.
Demonstrates: Cells are resistant to Ampicillin when transformed with the mixture of three plasmids (pChromoBlue, pChromoPink, pChro- moPurple). The colored proteins are not produced in the absence of IPTG.
Incorporating STEAM into Your Transformation Experiment Art, design, and innovation are key components to a bal- anced science education. Incorporating Art into your STEM curriculum can be a fun way to engage students and build a truly integrated curriculum. In this simple STEAM experiment the bacteria previously transformed by your students will become living paint for their artwork. All You Need: • ReadyPour™ Luria Broth Agar Base with Ampicillin Cat. #616 • Large Petri Plates Cat. #643 • IPTG Cat. #613 • Luria Broth Media Cat. #611 • Sterile Loops Cat. #667 Brief Experimental Procedure Each student or student group will share the transformed bacterial plate. 1. Using a sterile loop, scrape bacteria off of the transformation plate and resuspend in 1 mL of LB or Recovery Broth in a snaptop tube. 2. Using the same loop, spread the resuspended bacteria onto a fresh LB/Amp/IPTG plate.
NOTE: Encourage students to sketch their design before streaking onto their plates.
3. Place the plates in an inverted position in a 37°C incubator overnight before viewing the results.
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