CAST24 • EDVOTEK® Workshops

05 - Biotechnology Basics: My First Electrophoresis

Target Sequence

3' 5'

5' 3'

Separation of two DNA strands

=

5'

3'

= Primer 1 = Primer 2

Denature 94°C

3'

5'

5'

3'

5'

Anneal 2 primers 40°C - 65°C

5'

3'

5'

5'

Extension 72°C

3'

5'

3'

5'

5'

5'

3'

5' 3'

5'

5'

5'

3'

5'

3'

5'

5'

5'

3'

5' 3' 5' 3' 3' 5' 5'

5'

5' 3' 5'

5'

5' 3' 5' 3' 5' 3'

3' 5' 5'

5'

3'

5'

Figure 2: Three-step PCR

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