CAST24 • EDVOTEK® Workshops

05 - Biotechnology Basics: My First Electrophoresis

Agarose Gel Electrophoresis, continued

9. PLACE the gel (on the tray) into the electrophoresis chamber. COVER the gel with 1X electrophoresis buffer (See Table B for recommended volumes). The gel should be completely submerged. 10. LOAD the entire sample (35 µL) into the well in the order indicated by the Gel Loading Table for your experiment. 11. CHECK that the gel is properly oriented, then PLACE the safety cover onto the chamber. Remember, the DNA samples will migrate toward the positive (red) electrode. 12. CONNECT the leads to the power source and PERFORM electrophoresis (See Table C for time and voltage guidelines). 13. After electrophoresis

is complete, REMOVE the gel and casting tray from the electrophoresis chamber and PROCEED to visualization.

1x Electrophoresis Buffer (Chamber Buffer)

Dilution +

EDVOTEK Model #

Total Volume Required

50x Conc. Buffer

Distilled Water

EDGE™

150 mL

3 mL

147 mL

M12

400 mL

8 mL

392 mL

M36

1000 mL

20 mL

980 mL

Time and Voltage Guidelines (0.8% Agarose Gel)

Electrophoresis Model

EDGE™

M12 & M36

Volts

Min/Max (minutes)

Min/Max (minutes)

150 125 100

10/20

20/35 30/45 40/60

N/A

15/25

58

Visit our website at www.edvotek.com

Made with FlippingBook flipbook maker