05 - Biotechnology Basics: My First Electrophoresis
Agarose Gel Electrophoresis, continued
9. PLACE the gel (on the tray) into the electrophoresis chamber. COVER the gel with 1X electrophoresis buffer (See Table B for recommended volumes). The gel should be completely submerged. 10. LOAD the entire sample (35 µL) into the well in the order indicated by the Gel Loading Table for your experiment. 11. CHECK that the gel is properly oriented, then PLACE the safety cover onto the chamber. Remember, the DNA samples will migrate toward the positive (red) electrode. 12. CONNECT the leads to the power source and PERFORM electrophoresis (See Table C for time and voltage guidelines). 13. After electrophoresis
is complete, REMOVE the gel and casting tray from the electrophoresis chamber and PROCEED to visualization.
1x Electrophoresis Buffer (Chamber Buffer)
Dilution +
EDVOTEK Model #
Total Volume Required
50x Conc. Buffer
Distilled Water
EDGE™
150 mL
3 mL
147 mL
M12
400 mL
8 mL
392 mL
M36
1000 mL
20 mL
980 mL
Time and Voltage Guidelines (0.8% Agarose Gel)
Electrophoresis Model
EDGE™
M12 & M36
Volts
Min/Max (minutes)
Min/Max (minutes)
150 125 100
10/20
20/35 30/45 40/60
N/A
15/25
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