CAST24 • EDVOTEK® Workshops

05 - Biotechnology Basics: My First Electrophoresis

Scenario A: Visualizing the Dye Gel

dH 2 O

IF VISUALIZING A DYE GEL: 1. SLIDE the gel off the casting tray onto the white viewing surface of the transil - luminator. Dyes should appear as colorful bands on a light background. 2. PHOTOGRAPH the results. 3. REMOVE and DISPOSE of the gel and CLEAN the transilluminator surfaces with distilled water.

PATERNITY TESTING SIMULATION USING DYE ELECTROPHORESIS

The low molecular weight band (red) in the child's DNA profile is not present in the mother's DNA profile, meaning that it must be contributed by the father. This band is only found in Father 1, suggesting he is the father.

Lane

Tube

Sample

Sizes

1

A

Standard Dye Marker

5000, 3000, 1000, 500

2

B

PCR control reaction

3250, 1000

3

C

PCR reaction from Mother DNA

5000, 850

4

D

PCR reaction from Father 1 DNA

3000, 550

5

E

PCR reaction from Father 2 DNA

3250, 1000

6

F

PCR reaction from Child DNA

5000, 550

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