05 - Biotechnology Basics: My First Electrophoresis
Scenario B: Visualizing the SYBR® Gel
dH 2 O
IF VISUALIZING A SYBR® GEL: 1. SLIDE the gel off the casting tray onto the blue viewing surface of the transil - luminator. Close the orange lid and turn the unit on. DNA should appear as bright green bands on a dark background. 2. PHOTOGRAPH the results. 3. REMOVE and DISPOSE of the gel and CLEAN the transilluminator surfaces with distilled water.
The DNA standards in Lane 1 make it possible to measure the DNA bands obtained from the PCR reactions. The results of this analysis indicates an identical pattern in Lanes 2 and 4. This is strong evidence that the crime scene DNA and Suspect 2 match. In criminal investigations, several known variable regions in DNA are analyzed to match crime scene and suspect DNAs.
Lane
Tube
Sample
Sizes
1
A
DNA Standard Marker
6751, 3652, 2827, 1568 1118, 825, 630
2
B
Crime scene PCR reaction
3000, 1282
3
C
Suspect #1 PCR reaction
3000
4
D
Suspect #2 PCR reaction
3000, 1282
5
E
Suspect #3 PCR reaction
3652, 630
60
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