05 - Biotechnology Basics: My First Electrophoresis
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Linking STEM to Agarose Gel Electrophoresis For 10 Lab Groups. Link important STEM concepts using Agarose Gel Electro- phoresis. Help your students learn about the application of gel electrophoresis in DNA Fingerprinting, DNA Paternity Testing, Genetics (related to health and well-being), and the detection of Genetically Modified Foods. These dyes can be separated in agarose gels and students will use core STEM tools to determine band size and utilize critical thinking and reasoning skills. Four unique module options are supplied.
• Learn the biology behind gel electrophoresis and polymerase chain reactions (PCR) • Perform agarose gel electrophoresis to separate different sized dye molecules • Load, run, and analyze five simulated DNA samples plus a simulated DNA ladder • Choose from four real world scenarios: crime scene, paternity test, GMO detection, or medical diagnosis Cat. #S-46 (Dye-based experiment) DNA Fingerprinting by PCR Amplification For 8 Gels. Forensic DNA fingerprinting has become a universally accepted crime-fighting tool. Recent advances use the polymerase chain reaction (PCR) to amplify human DNA obtained from crime scenes. This experiment, based on a crime scene scenario, has an inquiry-based component. • Learn about DNA fingerprinting while solving a crime! • Introduce the biotechnologies of restriction enzyme digestion, PCR, and electrophore- sis • Perform agarose gel electrophoresis to separate differently-sized DNA molecules • Load, run, and analyze Ready-to-Load™ PCR reactions obtained from suspects and the crime scene. Cat. #130 (with FlashBlue™ Stain) Cat. #130-S (with SYBR® Safe DNA Stain)
SYBR® Safe DNA Stain Save time, money, the environment...and get better gel results! SYBR Safe® is a DNA stain that fluoresces with a bright green color when excited with blue light. SYBR® Safe binds specifically to the DNA double helix and has been engineered to be less muta- genic then Ethidium Bromide, making it much safer to use.
• SAFE for the Biotechnology Classroom • More sensitive than ethidium bromide • Non-mutagenic • 10,000 X Concentrate for 750 mL Cat. #608 (80 µL of SYBR® Safe Stain, )
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