TriFusion Blood Therapy (03/UV/LED)

TriFusion Blood Therapy (03/UV/LED) Advanced Regenerative Blood Therapy at Longevity Medical Institute

How the TriFusion System (O3/UV/LED) Works

Step 7: Return of Purified Bioactivated Blood

Step 6: LED Light (Photobiomodulation)

Step 5: Ultraviolet (UV) Irradiation

Step 1: Blood Extraction (Catheter to Pump)

TriFusion Device

Step 3: Ozonation (O3 Infusion)

Step 8: Systemic Eects and Integration Completed

Step 2: Blood Enters EBOO Filter

Step 4: Ozonated Blood Leaves EBOO Filter

Overview At Longevity Medical Institute, we offer the most advanced regenerative medical technologies available, including our TriFusion Blood Therapy, the first and only in Los Cabos, combining three powerful integrated modalities: Extracorporeal Blood Oxygenation and Ozonation (EBOO), Full Spectrum Ultraviolet Blood Irradiation (UVBI), and LED Blood Irradiation (LED-BI).

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This unique approach detoxifies, oxygenates, and rejuvenates the blood while delivering photobiological stimulation to the immune and circulatory systems. The result is a therapy that activates healing at the cellular level—enhancing mitochondrial function, reducing inflammation, eliminating pathogens, and supporting immune resilience. EBOO Full Spectrum UV at Longevity Medical Institute is a high-volume, closed-loop blood treatment capable of processing up to 3.5 liters of blood (nearly 2/3 of the blood in your body) per session—far exceeding the reach of traditional ozone IV therapy. Our treatment platform is optimized for clinical precision, patient safety, and maximum therapeutic yield. What Makes TriFusion at LMI Unique

How the Treatment Works

Blood Extraction (Catheter to Pump) Step 1:

A sterile, closed-loop circuit is established by inserting two intravenous (IV) catheters—one in each arm.

Blood Enters EBOO Filter Step 2:

The patient’s blood flows through a specialized EBOO filter, which acts similarly to a dialysis membrane.

This step removes toxic byproducts, excess cytokines, cellular debris, lipid peroxides, and other inflammatory mediators while preserving beneficial components of the blood.

Ozonation (O Infusion) Step 3:

Medical-grade ozone (a gas composed of three oxygen atoms) is carefully infused into the extracorporeal blood in controlled concentrations.

Ozone interacts with blood components to:

Break down pathogens (bacteria, viruses, fungi). Enhance red blood cell flexibility and oxygen-releasing capacity. Activate antioxidant defenses by stimulating Nrf2 pathways. Trigger immune modulation through mild oxidative signaling.

Ozonated Blood Leaves EBOO Filter Step 4:

Ultraviolet (UV) Irradiation Step 5:

The ozonated and oxygenated blood then passes through a UV light chamber, typically utilizing UV-A and UV-C wavelengths.

UV exposure helps:

Destroy pathogens Enhance immune recognition of abnormal or infected cells.

Oxidize toxins and activate white blood cells for enhanced systemic immune response. Improve microcirculation and reduce hypercoagulability.

LED Light Therapy (Photobiomodulation) Step 6:

After UV treatment, the blood is exposed to LED lights emitting specific wavelengths (e.g., red, blue, green, and infrared).

This step promotes:

Mitochondrial activation in white and red blood cells (via cytochrome c oxidase stimulation). Reduction in oxidative stress and inflammation. Vascular relaxation and improved nitric oxide (NO) signaling. Photobiomodulation benefits at the cellular level, supporting regeneration and detoxification.

Return of Purified, Bioactivated Blood Step 7:

The treated blood, now enriched with oxygen, modulated by ozone, and energized by light, is reinfused into the patient’s body through the second IV line.

This process typically takes 45–60 minutes per session.

Systemic Effects and Integration Step 8:

Once reinfused, the blood begins exerting systemic effects:

Improved oxygen delivery to tissues. Decreased inflammation and pain. Immune balancing (downregulating autoimmunity or upregulating suppression of infections). Enhanced detoxification capacity at the liver and cellular levels. Mitochondrial support and rejuvenation, which may assist in chronic fatigue, infections, vascular issues, and neurodegeneration.

Therapeutic Technology and Mechanism of Action

01 Extracorporeal Blood Oxygenation and Ozonation (EBOO)

EBOO draws up to 3.5 liters of blood —up to two-thirds of total blood volume—and runs it through a closed-loop dialysis filter system, where a controlled blend of medical-grade ozone and oxygen is infused. The blood is returned to the patient in real-time, making this an active, continuous system. Unlike traditional ozone IV methods (MAH, 10-pass), EBOO offers greater volume treatment with enhanced detox, oxygenation, and immune modulation benefits.

The Role of the Filter in EBOO The filter used in EBOO therapy is a high-permeability hollow-fiber membrane, similar to those used in dialysis. Its function is critical for safe and effective ozone delivery:

Allows ozone and oxygen gases to interact with blood across a semipermeable membrane without introducing gas bubbles. Diffusion Surface:

Gases flow in the opposite direction of the blood to maximize exchange efficiency. Cross-Current Flow:

Converts ozone into dissolved forms (ozonides), avoiding gas embolism and enabling direct immune and antioxidant stimulation. Safety:

Large surface area supports high blood volume treatment in a relatively short session. Enhanced Exposure:

This filter technology is what distinguishes EBOO from standard ozone therapies—it allows your blood to be treated safely and thoroughly in one session.

Mechanisms of Ozone

Triggers hormetic oxidative stress that activates Nrf2, a transcription factor that boosts antioxidant defenses and detoxification pathways.

Modulates immune signaling through lipid ozonation products (LOPs) and indirect stimulation of cytokines.

Enhances mitochondrial function, leading to better ATP production.

Kills microbes in the extracorporeal loop through direct oxidation.

02 Ultraviolet Blood Irradiation (UVBI)

Following ozonation, the blood passes through a chamber exposed to full-spectrum UV light—UVA, UVB, and UVC, plus visible LED light frequencies.

Mechanisms of UV Light

Direct inactivation of microbes via DNA/RNA disruption (especially UVC).

Induces hormetic response that boosts antioxidant enzymes.

Modulates immune function through toll-like receptor activation and nitric oxide (NO) upregulation.

Photobiomodulation stimulates tissue repair and inflammation resolution.

Visible Light LED Benefits

Full-spectrum LED strips stimulate additional photobiological effects:

Red and infrared: Enhance mitochondrial health and circulation Blue and violet: Antimicrobial and anti-inflammatory properties Green and yellow: Skin and tissue rejuvenation

Conditions Supported

Neurological & Cognitive Alzheimer’s, Parkinson’s, MS, ALS, neuropathies, migraines, TBI, post-stroke, Long COVID, brain fog

Autoimmune & Inflammatory Rheumatoid arthritis, lupus, Hashimoto’s, IBD, fibromyalgia, chronic inflammatory syndromes

Infectious & Toxic Load Chronic viral infections (EBV, CMV, HSV, HPV), Lyme disease, mold toxicity, heavy metal toxicity, CIRS

Cardiovascular & Metabolic Atherosclerosis, hypertension, diabetes, wound healing issues, metabolic syndrome

Musculoskeletal & Pain Syndromes Osteoarthritis, tendonitis, bursitis, back and neck pain, post-surgical inflammation

Longevity & Optimization Mitochondrial support, immune tuning, detoxification, performance recovery, biohacking, anti-aging

What to Expect During Therapy

Before Your Session Eat a balanced meal with protein and healthy fats Stay well hydrated to enhance vein access and therapeutic outcomes

During Your Session You’ll relax comfortably while your blood is withdrawn, ozonated, illuminated, and returned simultaneously

After Your Session No downtime—resume normal activities immediately

You may feel energized, relaxed, or experience detox-like fatigue—responses vary Results are cumulative; benefits increase over time with consistent application

Treatment Frequency at Longevity Medical Institute

Each protocol is personalized based on your condition, diagnostic markers, and treatment response. However, the following guidelines provide a foundational framework:

Chronic Illness, Neurological Disorders, Auto-Immune Conditions, or General Immune Dysregulation 1 session per week for 3–5 weeks Progress is reevaluated based on labs and symptom improvement Optional Maintenance: 1 session every 4–6 weeks

Wellness, Longevity & Performance 1 session every 4 weeks Ideal for detoxification, mitochondrial support, recovery, and ongoing optimization

These frequencies may be adjusted depending on progress, symptom resolution, and individual goals. Safety and Contraindications Acute Immune Recovery 1–2 sessions over a 1–2 week period Beneficial for rapid immune support following infections or inflammatory stressors EBOO 03UV is extremely well-tolerated when administered by trained professionals. At Longevity Medical Institute, we perform full clinical evaluations to ensure safety and efficacy.

Contraindications

G6PD deficiency Uncontrolled hyperthyroidism Seizure disorders Severe cardiovascular instability Thrombocytopenia Porphyria (specific to UV light sensitivity)

Optional biomarker panels measured in the in-house LMI Clinical Lab (e.g., CRP, oxidative stress markers, immune panels) may be used to track progress.

EBOO Full Spectrum UV reflects our belief that true healing occurs at the cellular level. As leaders in regenerative and anti-aging medicine, we integrate this therapy as a core part of our advanced treatment protocols, often alongside: Why We Offer TriFusion at LMI

Stem Cells and Exosomes Peptide therapies IV nutrient drips Hyperbaric oxygen therapy (HBOT) Longevity Recharge Station: 10 wave length PBM light, molecular hydrogen inhalation, and more Clinical lab diagnostics

Whether you're facing chronic illness or simply want to feel and function at your peak, TriFusion is a cornerstone in our personalized care ecosystem.

Contact Longevity Medical Institute to learn more about how personalized TriFusion therapy can help you stay at your peak for years to come.

Paseo Malecon San Jose 137, Zona Hotelera, 23405 San José del Cabo, B.C.S., Mexico Call or SMS (US & Canada): 1-833-LMI-CABO WhatsApp International: +52 624 237 1594 Visit: longevity-institute.com Contact Us

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Important Disclosure In San Jose del Cabo (B.C.S), Mexico, where we conduct these treatments, our clinic operates under the regulatory oversight of COFEPRIS (Comisión Federal para la Protección contra Riesgos Sanitarios), which is Mexico's equivalent of the U.S. Food and Drug Administration (FDA). COFEPRIS ensures the safety, efficacy, and quality of medical treatments and healthcare practices. Our clinic is licensed by COFEPRIS, guaranteeing that our treatments meet the highest standards of safety and efficacy. While our stem cell and other therapies are approved by COFEPRIS, they are not approved by the U.S. Food and Drug Administration (FDA). Patients considering these treatments should be aware of this distinction. As with any medical treatment, there are risks involved, and individual outcomes may vary. It is important to consult with your physician, thoroughly research the therapies, and carefully consider these risks before undergoing any stem cell or regenerative therapy. Disclaimer The information provided on this site is for informational purposes only and should not be considered medical advice. This site is not intended for the promotion or solicitation of services to audiences where such promotion may conflict with local regulations, including the United States and Canada. If you have questions or concerns about your health, please consult a qualified healthcare professional. All therapies and treatments discussed are subject to applicable laws and regulations and may not be available in all regions. We encourage all patients to conduct their own research, consult with their healthcare providers, and make informed decisions based on the best available information.

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