MOF-enzyme biocomposite as a potent tool for organic compounds oxidation and degradation Iltaf Shah 1 , Khadega AlMaqdi 1 , Salman Ashraf 2 1 UAE University, United Arab Emirates, 2 Khalifa University, United Arab Emirates The increase in the global population in recent years has caused a fast increase in pollution problems worldwide. One of these problems is emerging pollutants [1] , which are a class of human-made organic compounds that have been detected in various water bodies [2] . To develop a recyclable, reusable, and efficient material for the degradation of these pollutants, we report the synthesis, characterization and application of metal-organic framework(UiO-66-NH 2 ) embedded with horseradish peroxidase enzymes. The metal-organic framework/ enzyme composite [3] has crystallized in a ball-shaped crystal, had a surface area of 207.3m 2 /g, and had a mean pore size of 2.73nm. When tested against a panel of diverse compounds, the UiO- 66-NH 2 /enzyme complex was able to degrade a number of emerging pollutants with and without the presence of a redox mediator, including 2-mercaptobenzothiazole (MBT), Paracetamol, Methylparaben, sulfamethoxazole and salicylic acid, with efficiencies of 99.7%, 97.4%, 57.6%, 53.1%, and 54.3%, respectively. Furthermore, we tested therecyclabilityof the UiO-66-NH 2 /enzyme complex and found that they could be reused for four cycles. Our results suggest that enzyme/metal-organic framework is a potent remediation agent for the degradation of a set of emerging pollutants. Also, the successful immobilization of horseradish peroxidase enzymes on UiO-66-NH 2 shown in the present study can allow for the efficient recycling of horseradish peroxidase enzymes for multiple degradation cycles leading to potential scaling up and building of abioreactor. References 1. Al-Maqdi, Khadega A., Nada Elmerhi, Khawlah Athamneh, Muhammad Bilal, Ahmed Alzamly, Syed Salman Ashraf, and Iltaf Shah. "Challenges and recent advances in enzyme-mediated wastewater remediation—a review." Nanomaterials 11, no. 11 (2021): 3124. 2. Calvo-Flores, Francisco G., Joaquín Isac-García, and Jose A. Dobado. Emerging pollutants: origin, structure, and properties . John Wiley & Sons, 2018. 3. Yaghi, O. M., O'Keeffe, M., Ockwig, N. W., Chae, H. K., Eddaoudi, M., & Kim, J. (2003). Reticular synthesis and the design of new materials. Nature, 423(6941), 705-714.
P64
© The Author(s), 2023
Made with FlippingBook Learn more on our blog