PROTEINS, ENZYMES, & CHROMATOGRAPHY
Cat. #308 Fixing Genes, Fighting Disease: A Hands-On CRISPR Lab
For 6 Groups. TThis DMD CRISPR Simulation Kit introduces students to gene editing by modeling the correction of a Duchenne Muscular Dystrophy (DMD)-like mutation in bacteria. Using CRISPR-based tools, students restore fluorescence to a mutated GFP gene, simulating therapeutic correction of the dystrophin gene.
The hands-on workflow includes DNA ligation, bacterial transforma - tion, and protein extraction, culminating in SDS-PAGE analysis to visu-
alize gene expression differences. Through this process, students learn how genetic mutations disrupt protein production and how CRISPR can reverse these effects. The experiment emphasizes real-world applications of CRISPR in treating human genetic disorders like DMD, providing both molecular biology skills and disease context. This immersive lab bridges fundamental biotechnology with advanced gene therapy concepts in an accessible and engaging way.
Storage: Some Components Require Freezer & Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #308 Components and Requirements.
Cat. #1110 Cell Types in the Brain
For 6 Groups Sharing 3 Polyacrylamide Gels. The brain is responsible for regulating almost everything within our body. It does this through a network of cells working together to function. The objective of the experiment is for students to examine the differences between cell types in the brain based on their profiles of proteins.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #1110 Components and Requirements.
Cat. #317 Western Blot Analysis (Polyacrylamide-based)
Electrophoresis 60 min. Blot overnight Detection 2.5 hours
For 6 Groups. In Western blot analysis, protein identification is based on antibody and antigen reactions. Pro - teins are separated on polyacrylamide gels and are transferred (blotted) to a nylon membrane. The membrane is exposed to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme. The membrane is then soaked in a substrate solution to develop the color reaction, which results in identifica - tion on the antigen protein band. The molecular weights of the visible bands are measured using prestained protein markers of known molecular weight. This kit does not require an electrotransfer apparatus.
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #317 Components and Requirements.
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