PROTEINS, ENZYMES, & CHROMATOGRAPHY
Cat. #1110 Cell Types in the Brain
For 6 Groups Sharing 3 Polyacrylamide Gels. The brain is responsible for regulating almost everything within our body. It does this through a network of cells working together to function. The objective of the experiment is for students to examine the differences between cell types in the brain based on their profiles of proteins.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #1110 Components and Requirements.
Cat. #317 Western Blot Analysis (Polyacrylamide-based)
Electrophoresis 60 min. Blot overnight Detection 2.5 hours
For 6 Groups. In Western blot analysis, protein identification is based on antibody and antigen reactions. Pro - teins are separated on polyacrylamide gels and are transferred (blotted) to a nylon membrane. The membrane is exposed to solutions containing primary antibody, followed by a secondary antibody coupled to an enzyme. The membrane is then soaked in a substrate solution to develop the color reaction, which results in identifica - tion on the antigen protein band. The molecular weights of the visible bands are measured using prestained protein markers of known molecular weight. This kit does not require an electrotransfer apparatus.
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #317 Components and Requirements.
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