PROTEINS, ENZYMES, & CHROMATOGRAPHY
2025 EDVOTEK ® RESOURCE GUIDE
HORIZONTAL PROTEIN ELECTROPHORESIS
Cat. #110 Molecular Weight Determination of Proteins (Agarose-based)
Gel Prep 40 min. Electrophoresis 60 min.
For 6 Groups. Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis. Because the protein standards and “unknowns” are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is EDVOTEK®’s formulation of protein grade agarose, which provides an alternative to the use of polyacrylamide gels.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #110 Components and Requirements.
Cat. #275 Simulation of HIV Detection by Western Blot (Agarose-based)
Electrophoresis 45 min. Blot overnight Detection 25 min.
For 6 Blots. The second assay used to confirm a positive HIV ELISA result is the Western Blot. Students separate protein samples from hypothetical patients on agarose gels, transfer the samples to a membrane and detect the simulated HIV proteins. This kit is an introductory level experiment. For a comprehensive advanced course, we recommend Cat. #317 .
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #275 Components and Requirements.
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