PROTEINS, ENZYMES, & CHROMATOGRAPHY
Cat. #255 Purification & Size Determination of Green & Blue Fluorescent Proteins
For 6 Groups. When bacteria are used to make medicinally useful proteins by transformation, the pro - tein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP will be used as an assay during their purification from an E.coli extract. The column fractions containing GFP or BFP will be identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electro - phoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
Packing/running column 45 min. Optional electrophoresis 60 min. Staining 30 min. Destaining 2 hours
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #255 Components and Requirements.
Cat. #277 Affinity Chromatography of Glucose Binding Proteins
For 10 Groups. In this experiment, students will prepare a seed extract from Jack Bean Meal, fractionate the extract by affinity chromatography, and elute the bound glucose binding protein. The presence of biological activity is determined by an immunoblot enzyme assay.
Requires 2 hours
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #277 Components and Requirements.
Cat. #113 Principles of Thin Layer Chromatography
Spotting Plates 20 min. TLC Separation 5 min.
For 8 Separations. This experiment introduces chromatographic theory and methods of thin layer chromatography. A mixture of dyes are sepa- rated on a cellulose-based TLC plate using two different solvent systems.
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #113 Components and Requirements.
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