2024-2025 EDVOTEK® Resource Guide

BIG IDEA AP BIOLOGY 1 BIG IDEA AP BIOLOGY 3

BIG IDEA AP BIOLOGY 2

ADVANCED PLACEMENT BIOLOGY

GENETICS AND INFORMATION TRANSFER BIG IDEA AP BIOLOGY 4

Cat. #AP09 INVESTIGATION 9 Biotechnology: Restriction Enzyme Analysis of DNA For 8 Gels/8 Lab Groups. Restriction enzyme digestion and size separation by agarose gel electrophoresis are essential research tools that helped launch the era of biotechnology. In this activity, students will use agarose gel elec - trophoresis to separate, analyze, and compare bacteriophage lambda DNA before and after

Cat. #AP07

INVESTIGATION 7

Cell Division: Mitosis and Meiosis

For 10 Groups. Students learn to identify and differentiate various stages in mitosis and meiosis. Onion root tips are stained to identify

digestion with Hin dIII and Eco RI restriction enzymes. Using lambda DNA cut with Hin dIII, students can also construct a standard curve and determine the molecular weights of the two other samples.

the various stages and duration of mitosis. Meiosis and Crossing Over in Sordaria are also demonstrated in this experiment. Students will also have an opportunity to analyze the mechanism involved with loss of cell cycle control in cancer.

ALSO Available: Cat. #AP09-C DNA Samples Only in Microtest Tubes, For 24 gels

Requires 45 min.

Requires 60 min.

Storage: Room Temperature Stable. Storage of Ready-to-Load QuickStrip™ samples in the Refrigerator is Recommended. CLICK HERE For Experiment #AP09 Components and Requirements.

Storage: Room Temperature. CLICK HERE For Experiment #AP07 Components and Requirements.

Cat. #223-AP08

INVESTIGATION 8

Biotechnology: Bacterial Transformation Transformation of E.coli with Green Fluorescent Protein

Cat. #AP08-AP09 SPECIAL AP08-AP09 Special Bundle Our Bestselling AP® Experiments are now in one easy mini bundle to make exploring inquiry-based biotechnology a snap! These kits include new enhanced protocols with slide presentations, fillable student lab note - book pages, and detailed instructor guides with practical tips to avoid pitfalls.

For 10 Groups. In this experiment, transformed cells take up a plasmid containing the GFP gene, which has been isolated from the

jellyfish Aequorea victoria . Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to long wave UV light.