ADVANCED PLACEMENT BIOLOGY
2026 EDVOTEK ® RESOURCE GUIDE
Cat. #109
INVESTIGATION 9 OPTION
DNA Fingerprinting by Restriction Enzyme Patterns
For 8 Groups. DNA fingerprinting examines highly variable regions of DNA in order to create a genetic profile of an individual. Criminal investigators use DNA fingerprinting to help identify individuals, place a person at a crime scene, or eliminate a suspect from consideration. Importantly, DNA fingerprinting has less than a one in a billion chance of matching another individual (except an identical twin) and is very difficult to fake or change. In this experiment, students use gel electrophoresis to compare crime scene DNA to the DNA of two suspects. Particular emphasis is placed on RFLP analysis, the power of restriction enzymes to detect DNA sequence dif - ferences, and the importance of combining evidence from multiple DNA locations.
RELATED EQUIPMENT
Cat. #5062 Classroom DNA Electrophoresis LabStation ™
Includes: 1 Cat. #515 M36 HexaGel™ Electrophoresis Apparatus 1 Cat. #509 DuoSource™ Power Supply (100/150 V for 1 or 2 units) 2 Cat. #588 Fixed Volume MiniPipette (40 µL) 1 Cat. #636 Yellow Micropipette Tips (1 - 200 µL / 2 Racks of 96) 1 Cat. #130 DNA Fingerprinting Classroom Kit
Cat. #212 INVESTIGATION 9 OPTION Cleavage of Lambda DNA with Eco RI Restriction Enzyme
For 10 restriction digestions and 5 gels. Restriction enzymes are endonucleases that catalyze the cleavage of DNA. This process can be observed using agarose gel electrophoresis - a powerful separation method frequently used in molecular biology. In this lab, the DNA from bacteriophage lambda is digested with the restriction enzyme Eco RI. Students will digest DNA, run agarose gel electrophoresis, size the fragments using a standard curve, and compare the fragments to undigested lambda DNA. A great way to teach key research tools that helped launch the era of biotechnology!
Cat. #852 INVESTIGATION 12 OPTION Chemotaxis: The Science of Attraction in C. elegans
For 10 lab groups. All organisms are affected by “scent” molecules in the environment, includ - ing a multicellular organism called Caenorhabditis elegans. These worms are composed of 959 somatic cells, of which 300 are neurons comprising organs for taste, smell, temperature and touch. In this experiment, your students will observe and record the phenomenon by which normal and mutant strains of C. elegans can direct their movement in response to certain chemicals in the environment. *Kit contains LIVE materials. Please request materials 2 weeks prior to lab*
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