2025 EDVOTEK® Resource Guide

DISCOVERING ELECTROPHORESIS

Cat. #S-53 Mystery of the Crooked Cell

Cat. #S-48 What is PCR & How Does It Work?

For 10 Gels/10 Lab Groups. This simple lab demonstrates detection of the mutation that causes Sickle Cell Anemia. In this simulation, your students will use electrophoresis to separate dyes that represent patient samples and controls.

For 10 Gels/10 Lab Groups. This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophore- sis. NGSS-aligned with MS-LS1.

Target Sequence

Separation of two DNA strands

=

3' 5'

5' 3'

Primer 1 = Primer 2 =

5'

3'

Denature 94°C

3'

5'

5'

3'

5'

Anneal 2 primers 40°C - 65°C

5'

3'

5'

Extension 72°C

5'

3'

5'

Developed in Partnership with CityLab Boston University School of Medicine

3'

5'

5'

5'

3'

5' 3'

Department of Health and Human Services • National Institutes of Health Supported by a Science Education Partnership Award (SEPA) from the National Center for Research Resources.

5'

5'

5'

3'

5'

3'

5'

5'

5'

3'

5' 3' 3' 3' 5' 5'

5'

5' 3' 5'

5'

5' 3' 5' 3' 5' 3'

3' 5'

5'

5'

5'

3'

5'

31

1.800.EDVOTEK • www.edvotek.com • info@edvotek.com

Made with FlippingBook - professional solution for displaying marketing and sales documents online