2024-2025 EDVOTEK® Resource Guide

READY-TO-LOAD™ DNA ELECTROPHORESIS

Cat. #AP09-112 Restriction Enzyme Analysis of DNA

For 8 Gels/8 Lab Groups. This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bacteriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.

ALSO Available: Full Experiment featuring SYBR® Safe DNA Stain DNA Samples Only in QuickStrips™ for 8 gels DNA Samples Only in Microtest Tubes for 24 gels

Cat. #AP09-112-S Cat. #AP09-112-A Cat. #AP09-112-C

Cat. #102 Restriction Enzyme Cleavage of Plasmid & Lambda DNA

For 8 Gels/8 Lab Groups. Plasmid and lambda DNA are pre-digested with restriction enzymes - endonucleases that recognize and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

5’ 3’

G A A T T C C T T A A G

3’ 5’

Eco RI

ALSO Available: Full Experiment featuring SYBR® Safe DNA Stain DNA Samples Only in QuickStrips™ for 8 gels DNA Samples Only in Microtest Tubes for 24 gels

Cat. #102-S Cat. #102-A Cat. #102-C

Cat. #105 Mapping of Restriction Sites on Plasmid DNA

For 8 Gels/8 Lab Groups. DNA mapping is a common procedure used to deter- mine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

ALSO Available: Full Experiment featuring SYBR® Safe DNA Stain DNA Samples Only in QuickStrips™ for 8 gels DNA Samples Only in Microtest Tubes for 24 gels

Cat. #105-S Cat. #105-A Cat. #105-C

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