2026 EDVOTEK® Resource Guide

ADVANCED DNA APPLICATIONS

Cat. #205 Bee-coming Royals: DNA Methylation of Honey Bees

For 6 Groups. In this experiment, students explore the effects of DNA methylation on restriction enzyme activity. Plasmid DNA will be digested with the restriction en - zymes Dpn I and Dpn II. When digested with these enzymes, methylated and unmeth-

ylated DNA will produce restriction fragments that are distinct from one another. The restriction fragments are then analyzed using agarose gel electrophoresis. After visualizing the gel, students determine which sample is methylated. Requires wet ice shipment for next day delivery (by 3:00 p.m. in most areas.)

Complete in 2 hours and 30 min. (with optional stopping points).

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #205 Components and Requirements.

Cat. #225 DNA Fingerprinting Using Restriction Enzymes

Cat. #212 Cleavage of Lambda DNA with Eco RI Restriction Enzyme

AP Biology Investigation #9 Option

For 10 Restriction Digestions and 5 Gels. The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes

For 6 Gels. Teach your students about restriction enzyme digests in the context of forensic science! Your students will cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.

and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analyzed by electrophoresis.

Complete in 1 hour and 20-45 min.

Complete in 90 min.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #225 Components and Requirements.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #212 Components and Requirements.

Cat. #206 Restriction Enzyme Mapping

For 6 Sets of Restriction Digestions. In this experiment, a plasmid DNA is cleaved with dif- ferent combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped.