2024-2025 EDVOTEK® Resource Guide

ADVANCED DNA APPLICATIONS

2024-2025 EDVOTEK ® RESOURCE GUIDE

Cat. #210 A-maize-ing Editing: Using CRISPR to Improve Crops

For 8 Gels. Explore cutting-edge biotechnology with this hands-on CRISPR-Cas9 simulation. Students will assume the role of plant geneticists working to develop corn crops capable of surviving, and thriving, in a changing environment. This experiment will allow students to develop an understanding of CRISPR-Cas9 applications in the laboratory, cleave DNA, and examine their results after gel electrophoresis.

Complete in 1.5 hours.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #210 Components and Requirements.

Cat. #205 Bee-coming Royals: DNA Methylation of Honey Bees

For 6 Groups. In this experiment, students explore the effects of DNA methylation on restriction enzyme activity. Plasmid DNA will be digested with the restriction enzymes Dpn I and Dpn II. When digested with these enzymes,

methylated and unmethylated DNA will produce restriction fragments that are distinct from one another. The restriction fragments are then analyzed using agarose gel elec- trophoresis. After visualizing the gel, students determine which sample is methylated. Requires wet ice shipment for next day delivery (by 3:00 p.m. in most areas.)

Complete in 2 hours and 30 min. (with optional stopping points).

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #205 Components and Requirements.

Cat. #202 Mini-Prep Isolation of Plasmid DNA

Cat. #203 Isolation of E.coli Chromosomal DNA

For up to 20 DNA isolations and 5 gels. Isolation of high molecular weight chromosomal DNA is the first step in

For up to 20 plasmid isolations for twelve 7x7 cm gels. Small-scale rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and subcloning experi- ments. In this experiment, students iso- late plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

molecular cloning since it is the source of genes in cells. This experiment provides DNA Extraction LyphoCells™ and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analyzed by agarose gel electrophoresis as an optional lab extension activity.

Isolation of Plasmid DNA 60 min. Electrophoresis 45 min.

Complete in 1 hour and 45 min.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #202 Components and Requirements.

Storage: Room Temperature. CLICK HERE For Experiment #203 Components and Requirements.

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