ADVANCED DNA APPLICATIONS
Cat. #300 Blue/White Cloning of a DNA Fragment & Assay of ß-galactosidase
For 5 Groups. When DNA is subcloned in the pUC polylinker region, ß-galactosidase production is inter- rupted, resulting in the inability of cells to break down X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA Ligase. Following the ligation to synthesize the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic resistant white and blue colonies are counted. ß-galactosidase activity is assayed from blue and white bacterial cells. This ex- periment can be broken down into three modules: ligation, transformation, and assay of ß-galactosidase.
Complete in 3 one-hour periods.
Cat. #300 is recommended for college level courses.
Storage: Some Components Require Refrigerator & Freezer Storage Upon Receipt. CLICK HERE For Experiment #300 Components and Requirements.
Cat. #957 Blinded by the Light: UV Rays and DNA Damage For 10 Groups. In this experiment, students directly observe the effects of UV light on DNA. Students will run a time series test comparing UV exposed plasmid samples and examine their results using electrophoresis. Students may also test the ability of different sunscreens to prevent DNA damage.
Complete in 2 hours.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #957 Components and Requirements.
Cat. #5062 Classroom DNA Electrophoresis LabStation™
Includes: 1 Cat. #515 M36 HexaGel™ Electrophoresis Apparatus 1 Cat. #509 DuoSource™ Power Supply (100/150 V for 1 or 2 units) 2 Cat. #588 Fixed Volume MiniPipette (40 µL) 1 Cat. #636 Yellow Micropipette Tips (1 - 200 µL / 2 Racks of 96) 1 Cat. #130 DNA Fingerprinting Classroom Kit
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