ADVANCED DNA APPLICATIONS
Cat. #300 Blue/White Cloning of a DNA Fragment & Assay of ß-galactosidase
For 5 Groups. When DNA is subcloned in the pUC polylinker region, ß-galactosidase production is inter- rupted, resulting in the inability of cells to break down X-Gal. This results in the production of white colonies amongst a background of blue colonies. This experiment provides a DNA fragment together with a linear plasmid and T4 DNA Ligase. Following the ligation to synthesize the recombinant plasmid, competent E. coli cells are transformed and the number of recombinant antibiotic resistant white and blue colonies are counted. ß-galactosidase activity is assayed from blue and white bacterial cells. This ex- periment can be broken down into three modules: ligation, transformation, and assay of ß-galactosidase.
Complete in 3 one-hour periods.
Cat. #300 is recommended for college level courses.
Storage: Some Components Require Refrigerator & Freezer Storage Upon Receipt. CLICK HERE For Experiment #300 Components and Requirements.
Cat. #957 Blinded by the Light: UV Rays and DNA Damage
For 10 Groups. According to the World Health Organization, between 2 and 3 million cases of skin cancer occur globally every year. Many of these cancers are caused by preventable damage
to DNA by UV light during sunbathing. In this experiment, students directly observe the effects of UV light on DNA. Students will run a time series test comparing UV exposed plasmid samples and examine their results using electrophoresis. The ability of various sunscreens to prevent DNA damage can also be tested.
INFRARED
VISIBLE
ULTRAVIOLET
1000-700 nm
700-400 nm
400-200 nm
UVA 400-320 nm
UVB 320-280 nm
UVC 280-200 nm
Complete in 2 hours.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #957 Components and Requirements.
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