POLYMERASE CHAIN REACTION
Cat. #341 Reeling in the Truth: Identifying Red Snapper using Genetic Analysis
For 24 reactions, or 6 groups of 4 students. Students will explore the genetic diversity of fish species in a simulated DNA barcoding experiment. Students will extract DNA from simulated fish samples and use PCR to amplify three gene regions that are conserved between species. • Learn how to extract DNA from simulated fish samples and amplify specific gene regions using PCR. • Understand how conserved gene regions can be used to identify species and study genetic diversity. • Explore DNA barcoding, a critical tool in species identification and conservation biology.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #341 Components and Requirements.
Cat. #343 ChickCheck: The Genetics Behind Blue Chicken Eggs
For 25 students working in 5 groups. Students will investigate the genetic basis of chicken egg color by using PCR to detect a viral insertion near the SLCO1B3 gene, which is responsible for the blue egg color. PCR products are then analyzed using electropho - resis to determine phenotypes and select breeding pairs that will produce homozygous blue egg-laying chickens.
• Explore the genetics of chicken egg color using hands-on PCR. • Detect a viral DNA insertion near the SLCO1B3 gene that causes blue eggs. • Compare PCR band patterns to identify egg-color phenotypes.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #343 Components and Requirements.
Cat. #958 Silent Signals: Tracking Disease Through PCR Wastewater Testing
For 25 students working in 5 groups. Wastewater testing is a valuable public health tool that allows scientists to monitor specific diseases within a community. In this experiment, students will use PCR and electrophoresis to analyze simulated water samples for the presence of bacteria and viruses. By exploring how wastewater monitoring works, students will gain insight into how biotechnology plays a vital role in protecting public health. • Students analyze simulated water samples using PCR and electrophoresis to investigate real-world wastewater surveillance. • Engaging, hands-on experience with essential biotechnology tools to detect and identify pathogens. • Illustrates how wastewater testing provides early detection of outbreaks and supports public health decision-making.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #958 Components and Requirements.
This project was made possible by a Science Education Partnership Award (SEPA), Grant Number 2R44GM143977-02, from the National Institute of General Medical Sciences (NIGMS) of the National Institutes of Health (NIH).
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