POLYMERASE CHAIN REACTION
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Cat. #345 Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR
For 25 Reactions. The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.
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Extraction 30 min. PCR Set Up 10 min. PCR 2 hrs. DNA Digest 60 min. Electrophoresis 30 min. Staining 5 min.
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For 25 Students. In this experiment, students will iso- late their mitochondrial DNA and use the Polymerase Chain Reaction (PCR) to amplify two separate regions of the mitochondrial genome. Results are analyzed using agarose gel electrophoresis. Cat. #332 The Mother of All Experiments: Exploring Human Origin by PCR Amplification of Mitochondrial DNA
Extraction 50 min. PCR Set Up 10 min. PCR 2 hrs. Electrophoresis 60 min. Staining 5 min. to overnight
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