POLYMERASE CHAIN REACTION
2024-2025 EDVOTEK ® RESOURCE GUIDE
Cat. #323 GFP Transformation Extension: Colony PCR
This kit is intended for use in conjunction with Edvotek Transformation Kits 222, 223 or 303.
For 10 Groups. Colony PCR represents a simple and easy way to determine whether cloning and transformation experiments were successful. In this ex- periment, students will use colony PCR to analyze bacteria transformed with pFluoroGreen. A single colony will be used as the DNA template for PCR. The resulting PCR sample will then be analyzed using agarose gel electrophoresis. If the bacteria have been transformed successfully, a PCR product represent- ing the GFP gene will be produced. A bacterial housekeeping gene is ampli - fied at the same time as a positive control. The presence of both bands is indicative of a successful transformation experiment.
Extraction 60 min. PCR Set Up 10 min. PCR 2 hrs. Electrophoresis 60 min. Staining 5 min. to overnight
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #323 Components and Requirements.
Cat. #331 Investigating Synthetic Biology
For 5 Groups. Teach your students about synthetic biology with this exciting and exclusive lab! Students use PCR to amplify the coding se- quence of the BSMT1 enzyme. This interesting enzyme is responsible for the formation of methyl salicylate, a chemical with a strong “winter- green” odor. The PCR product is purified, restriction enzyme digested, and inserted into a plasmid vector. The resulting recombinant DNA is then used to transform E. coli BactoBeads™. Finally, students design an experiment to express the enzyme from their transformants and per- form a smell test to confirm that the bacterial factories are working!
Module I: 2 hours Module II: 60 minutes Module III: 2 hours Module IV: 75 minutes
Module V: 2 hours + overnight Module VI: 3 hours 20 minutes + overnight Module VII: 15 minutes *This experiment requires a multiple day transformation.
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #331 Components and Requirements.
Cat. #372 Quick PCR
PCR Set Up 10 min. PCR 30 min. Electrophoresis 30 min. Staining 5 min. to overnight
For 10 Groups. In this experiment, students will gain an understanding of the traditional three-step Polymerase Chain Reaction (PCR). Using PCR and Agarose Gel Electrophoresis, they will analyze a small section of Lambda DNA in a time-saving two-step process.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #372 Components and Requirements.
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