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2026 EDVOTEK® Resource Guide

POLYMERASE CHAIN REACTION

2026 EDVOTEK ® RESOURCE GUIDE

Cat. #380 Discovering Quantitative PCR Amplification and Analysis

For 4 Groups. Quantitative PCR (qPCR, also known as real time PCR) simultaneously amplifies and detects targeted DNA allowing scientists to discover the starting amount of a specific DNA sequence in an experimental sample. This powerful and precise technol - ogy has become a cornerstone of modern genetics research, biological monitoring, and medical diagnostics. For students, performing qPCR offers the challenge of performing a mathematically and technically advanced experiment, the opportunity to understand key molecular concepts through hands on learning, and an essential skill for future research work. In this specially adapted education qPCR experiment, students will quantify the DNA concentration of four experimental samples using a standard curve approach and then confirm the experiment’s specificity and accuracy through gel electrophoresis, melt curve analysis, and data analysis. This kit must be used with a RT (qPCR) Thermal Cycler.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #380 Components and Requirements.

Cat. #381 Break Through! Testing DNA Damage Using Quantitative PCR

For 4 Groups. The integrity and stability of DNA is essential to life. However, everyday this molecule is under assault from environmental stressors like UV radiation, mutagenic chemicals, and even normal metabolic processes. In this guided inquiry lab students will use the cutting edge technology of QPCR to investigate and quantify DNA damage due to physical (UV radiation) or chemical (DNAseI) disruptions. By designing and performing the experiments students will master advanced analytical and technical skills as well as deepen their understanding of key molecular biology and medical concepts. This kit must be used with a RT (qPCR) Thermal Cycler.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #381 Components and Requirements.