2024-2025 EDVOTEK® Resource Guide

GENETIC ENGINEERING & TRANSFORMATION

2024-2025 EDVOTEK ® RESOURCE GUIDE

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Cat. #307 Code Breakers: Using CRISPR to Rewrite Genetics

Cat. #310 Hack the Planet: Using CRISPR to Terraform Mars

For 5 Groups. In this experiment, students will engineer bacteria that are capable of surviving on a distant planet! Students will simulate the use of CRISPR-Cas9 to modify bacterial DNA, which will then be transformed into auxo- trophic E. coli that are incapable of surviving on the Martian surface. Only bacteria that receive the successfully edited DNA can survive, thrive, and help to terraform Mars!

For 10 Groups. Unlock the exciting world of gene editing and inves- tigate the incredible power of CRISPR technology right from your classroom! In this experiment, students explore cutting-edge genetic engineering using CRISPR-Cas9 to knock out the purple chromogenic genes in classroom safe bacteria. Additionally, students test the specificity of CRISPR-Cas9 first hand by using a non-specific CRISPR gRNA templates and analyzing the results.

• Explore genetic engineering, CRISPR, and the central dogma of molecular biology in this comprehensive experiment. • Transform classroom-safe E. coli with CRISPR RNA templates to test the targeting specificity of the technology. • Identify successful gene knockout based on the color of the cells.

Complete in three 1-hour modules.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #310 Components and Requirements.

Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #307 Components and Requirements.

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