GENETIC ENGINEERING & TRANSFORMATION
2025 EDVOTEK ® RESOURCE GUIDE
Cat. #223-AP08 Transformation of E.coli with Green Fluorescent Proteins (GFP)
Set Up & Plating 50 min. Incubation overnight Transformation 15 min.
For 10 Groups. In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analyzing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.
EDVOTEK ® enhanced transformation protocol included!
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Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #223-AP08 Components and Requirements.
Cat. #222 Transformation of E.coli with Blue and Green Fluorescent Proteins
Complete in 50 min. and grow overnight
For 10 Groups. The Green Fluorescent Protein (GFP), which is responsible for biolumines - cence in the jellyfish Aequorea victoria , is used extensively in all areas of science. Many organ- isms have been transformed with the GFP gene. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment your students will transform bacteria either with GFP, BFP or both!
EDVOTEK ® enhanced transformation protocol included!
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #222 Components and Requirements.
Cat. #323 GFP Transformation Extension: Colony PCR
This kit is to be used in conjunction with EDVOTEK® Cat. #222, #223, or #303.
For 10 Groups. Colony PCR represents a simple and easy way to determine whether cloning and transformation experiments were successful. In this experiment, students will use colony PCR to analyze bacteria transformed with pFluoroGreen. A single colony will be used as the DNA template for PCR. The resulting PCR sample will then be analyzed using agarose gel electrophoresis. If the bacteria have been transformed successfully, a PCR product representing the GFP gene will be produced. A bacterial housekeeping gene is amplified at the same time as a positive control. The presence of both bands is indicative of a successful transformation experiment.
Extraction 60 min. PCR Set Up 10 min. PCR 2 hrs. Electrophoresis 60 min. Staining 5 min. to overnight
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #323 Components and Requirements.
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