GENETIC ENGINEERING & TRANSFORMATION
Cat. #221 Transformation of E.coli with pGAL ™ (Blue Colony)
For 10 Groups. In this experiment, your students will develop an understanding of bacterial transformation of plasmid DNA by introducing an opportunity to observe an acquired phenotypic trait of the transformed bacterial cells. The presence of blue bacterial colonies visually demonstrates the expression of a specific gene for the Lac+ phenotype.
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #221 Components and Requirements.
Complete in 50 min. and grow overnight
Cat. #224 Rainbow Transformation
EDVOTEK ® enhanced transformation protocol included!
For 10 Groups. Transformation is of central importance in molecular cloning since it allows for the selection, propagation, expression and purification of a gene. Positive selection for cells containing plasmid DNA is accomplished by antibiotic growth selection. In this experiment, your students will transform bacteria with a new set of rainbow color plasmids that transform non-pathogenic bacterial cells into bright, colorful cells.
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #224 Components and Requirements.
Set Up & Plating 50 min. Incubation overnight Transformation efficiency 15 min.
Cat. #331 Investigating Synthetic Biology
For 5 Groups. Teach your students about synthetic biology with this exciting and exclusive lab! Students use PCR to amplify the coding sequence of the BSMT1 enzyme. This interesting enzyme is responsible for the formation of methyl salicylate, a chemical with a strong “wintergreen” odor. The PCR product is purified, restriction digested, and inserted into a plasmid vector. The resulting recombinant DNA is then used to transform E. coli BactoBeads™. Finally, students design an experiment to express the enzyme from their transformants and perform a smell test to
confirm that the bacterial factories are working! Cat. #331 is recommended for college level courses.
Module I: Amplification by PCR 2 hours or overnight Electrophoresis 45 to 60 min. Module II: Preparation for Ligation 90 to 110 min. Module III: Ligation into vector 40 to 70 min.
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #331 Components and Requirements.
Module IV: Transformation 30 to 45 min. and overnight incubation Module V: Smell Assay 15 to 30 min. and overnight incubation *This experiment requires a multiple day transformation.
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