GENETIC ENGINEERING & TRANSFORMATION
2026 EDVOTEK ® RESOURCE GUIDE
Cat. #255 Purification & Size Determination of Green & Blue Fluorescent Proteins
Packing & running column 45 min. Optional electrophoresis 60 min. Staining (optional) 30 min. Destaining (optional) 2 hours/overnight
For 6 Groups. When bacteria are used to make medicinally useful proteins by transformation, the protein of interest must be separated from all of the other cellular proteins. In this experiment, the unique fluorescent properties of GFP and BFP will be used during their purification from an E.coli extract. The column fractions containing GFP or BFP will be identified by fluorescence and then purified. As an optional activity, purified protein fractions can be separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the purity and size of the GFP and BFP proteins.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #255 Components and Requirements.
Cat. #304 The Future of Biofuels: Alcohol Fermentation
Complete over multiple lab periods.
For 5 Groups. Ethanol fermentation is the most common method for biofuel production worldwide. In this experiment, students will use small- scale flask fermenters to quantify ethanol production and sugar utilization by Saccharomyces cerevisiae . By controlling variables such as temperature and aeration, the students can compare the efficiency of the fermenta - tions over a three day experiment.
Storage: Some Components Require Refrigerator and Freezer Storage Upon Receipt. CLICK HERE For Experiment #304 Components and Requirements.
Cat. #121 Detection of Genetically Modified Organisms
Complete in 60 minutes
For 8 Gels/8 Lab Groups. For centuries, humans have used selective breeding and conventional hybridiza- tion to produce desirable qualities and to increase crop yields. Today, scientists use genetic engineering to directly manipulate the DNA, quickly producing these desirable traits. In this experiment, students will use agarose gel electrophoresis to explore the molecular methods used by scientists to identify geneti- cally modified organisms. No thermal cycler is required. Students are also encouraged to explore the pros and cons surrounding the use of genetically modified organisms.
Storage: Room Temperature Stable. Storage of Ready-to-Load™ QuickStrip™ samples in the Refrigerator is Recommended. CLICK HERE For Experiment #121 Components and Requirements.
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