FORENSICS
2024-2025 EDVOTEK ® RESOURCE GUIDE
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Cat. #190 Forensic Escape Room: Design Your Own Biotech Adventure
What is a DNA Fingerprint? If we analyze the polymorphisms (small differences in the DNA sequence) within a person’s genome, we can generate a unique “DNA fingerprint.” After DNA is extracted from biologi - cal samples, scientists use the polymerase chain reaction (PCR) to amplify specific places (loci) throughout the genome. The PCR products are analyzed using agarose gel electrophoresis. The PCR products appear on the gel as a series of bands with various sizes. Because DNA samples from different individuals produce different patterns of bands, scientists can use a DNA fingerprint to distinguish between individuals. The best-known application of DNA fingerprinting is in forensic science. DNA fingerprinting techniques are utilized to analyze blood, tissue, or fluid evidence collected at accidents and crime scenes. The DNA fingerprint from a crime scene can be com - pared with the DNA fingerprints of different suspects or those stored in CODIS (COmbined DNA I ndex System), a computer da - tabase of DNA fingerprints collected from convicted offenders, arrested persons, crime scene evidence and missing persons. A match between the crime scene DNA and a suspect’s DNA at a single locus does not prove guilt, nor does it rule out innocence. Therefore, multiple loci are tested. For example, the DNA finger - prints stored in CODIS contain data on thirteen loci. The odds of a match at all thirteen loci are less than one in a trillion! Using our experiments, your students will compare “crime scene” DNA with “suspect” DNA! Try DNA Fingerprinting by PCR Amplification (Kit #130), DNA Fingerprinting Using Restriction Enzymes (Kit #225), or DNA Fingerprinting Using PCR (Kit #371).
For 10 Lab Groups. Explore the world of forensic science with this fun and exciting crime scene escape room! In this investigation, students decipher clues, solve puzzles, and unravel the evidence to free the innocent. Hands-
QuickStrips ™
on techniques include forensic blood detection, blood typing, and DNA fingerprinting. Comprehensive instructions on how to set up an escape room are included.
• Students analyze evidence using real-world forensic science techniques, like DNA analysis and blood typing, to prove a suspect’s innocence in this immersive scenario. • Students gain experience with biotechnology skills, including micropipet - ting, blood typing, and agarose gel electrophoresis. • The sequence of experiments supports critical thinking, collaboration, and application of scientific practices, such as data collection and analy - sis, in a way that aligns with NGSS high school life science performance expectations.
Complete in 60 min.
Storage: Some components require refrigerator storage. CLICK HERE For Experiment #190 Components and Requirements.
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