BIOMEDICAL SCIENCES
- Cancer
2024-2025 EDVOTEK ® RESOURCE GUIDE
Cat. #957 Blinded by the Light: UV Rays and DNA Damage
For 10 Groups. In this experiment, students directly observe the effects of UV light on DNA. Students will run a time series test comparing UV exposed plasmid samples and examine their results using electrophoresis. Students may also test the ability of different sunscreens to prevent DNA damage.
INFRARED
VISIBLE
ULTRAVIOLET
1000-700 nm
700-400 nm
400-200 nm
UVA 400-320 nm
UVB 320-280 nm
UVC 280-200 nm
Complete in 2 hours.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #957 Components and Requirements.
Cat. #314 In Search of the Cancer Gene
For 6 Groups. Suppressor genes such as p53 are essential for cell func- tions. Mutations in the p53 gene can be correlated to predisposition for certain cancers. Mutations in genes can either be inherited or ac- cumulated due to environmental insults. This experiment deals with a family pedigree determination of several generations relating to cancer formation due to p53 gene mutation. This experiment does not contain human DNA.
Complete in 1 hour.
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #314 Components and Requirements.
Cat. #381 Break Through! Testing DNA Damage Using Quantitative PCR
For 4 Groups. The integrity and stability of DNA is essential to life. However, everyday this molecule is under assault from environmental stressors like UV radiation, mutagenic chemicals, and even normal metabolic processes. In this guided inquiry lab students will use the cutting edge technology of QPCR to investigate and quantify DNA damage due to physical (UV radiation) or chemical (DNAseI) disruptions. By designing and performing the experiments students will master advanced analytical and technical skills as well as deepen their understanding of key molecular biology and medical concepts. This kit must be used with a RT (qPCR) Thermal Cycler.
DNA Damage - 45 min. qPCR Setup 45 min. qPCR - 90 min. Electrophoresis - 60 min. Staining - 5 min. to overnight Analysis - 45 min.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #381 Components and Requirements.
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