BIOMEDICAL SCIENCES
- Infectious Diseases
Cat. #271 AIDS Kit I: Simulation of HIV Detection by ELISA
Requires 1 hour.
For 10 Groups. An HIV test detects HIV infection indirectly using an ELISA test against HIV antibod- ies in the blood. The test works by taking antibodies from the patient’s blood and adding them to a microtiter plate coated with HIV antigen. If HIV antibodies are present in the blood, they will bind to the antigens on the plate. This binding is detected with an enzyme-linked secondary an- tibody that causes a color change upon addition of substrate. In this experiment, your students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #271 Components and Requirements.
Cat. #275 AIDS Kit II: Simulation of HIV Detection by Western Blot
For 6 Groups. The Human Immunodeficiency Virus (HIV) causes acquired immune deficiency syndrome (AIDS), a serious disease that suppresses a patient’s immune system which leaves them suscep- tible to infections. In this experiment, students will use SDS-PAGE Cat. #151 AIDS Kit III: Simulation of HIV Detection by Protein Electrophoresis
For 6 Groups. One assay used to confirm a positive HIV ELISA result is the Western Blot. Students separate protein samples from hypotheti- cal patients on agarose gels, transfer the samples to a membrane and detect the simulated HIV proteins. This kit is an introductory level experiment. For a comprehensive advanced course, we recommend Cat. #317.
to simulate the identification of HIV proteins in simulated patient samples. The results of this test are used to diagnose an HIV infection.
Electrophoresis 60 min. Staining/destaining (optional) 2 hours.
Electrophoresis 45 min. Blot overnight Detection 25 min.
Storage: Some Components Require Freezer Storage Upon Receipt. CLICK HERE For Experiment #151 Components and Requirements.
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #275 Components and Requirements.
Cat. #160 Identification and Characterization of Bacteria
Complete in 90 min.
For 8 Groups. Gram staining is a quick, effective, and medically relevant identification test that has become one of the most essential tools in bacterial classification. In this experi - ment, students will use this staining technique to examine the size, shape, arrangement, and gram status (+/-) of Escherichia coli, Bacillus subtilis, and Micrococcus luteus .
Storage: Some Components Require Refrigerator Storage Upon Receipt. CLICK HERE For Experiment #160 Components and Requirements.
89
1.800.EDVOTEK • www.edvotek.com • info@edvotek.com
Made with FlippingBook - professional solution for displaying marketing and sales documents online